Plenary Abstracts

Name Title
Chris Abell Fragments and droplets - developing new tools for enzymology. [doc]
Marc Fontecave DNA repair and free radicals: the case of the spore photoproduct lyase, a radical-SAM enzyme. [doc]
Dick Janssen Halohydrin dehalogenases: catalytic mechanism, engineering and biocatalytic applications. [doc]
Judith Klinman Tunneling and dynamics in enzymes : peeking under the covers of catalysis. [doc]
Vern Schramm Enzymatic transition states: cancer, autoimmunity, malaria and antibiotics. [doc]
Steve Withers Engineering and evolution of old enzymes for new tasks: glycoside assembly. [doc]
Sunney Xie Single molecule enzymology. [doc]

Speaker Abstracts

Name Title
Karen Allen Fitness through simplicity: dominance of the HAD phosphotransferase family. [doc]
Tadhg Begley Thiamin biosynthesis: still yielding fascinationg biological chemistry [doc]
Jean Boutin Our story of autotaxin: a typical example from discovery to biochemical characterization and inhibitor discovery. [doc]
David Merkler Redox-dependent inactivation of peptidylglycine amidationg monooxygenase by cinnamate and phenylbutenoates. [doc]
Teresa Fitzpatrick Vitamin B6 biosynthesis: mechanistic insights derived from structural and functional aspects of the participating proteins. [doc]
Neil Kelleher Linked studies of secondary metabolism using small and large molecule mass spectrometry. [doc]
Peter Leadlay Stereochemical aspects of polyketide antibiotic biosynthesis. [doc]
Marc Leibundgut Structure of fungal fatty acid synthase: a 2.6 MDa molecular assembly line. [doc]
Hung-wen Liu Mechanistic studies of novel biosynthetic enzymes. [doc]
Thierry Meinnel Sampling the conformational states involved in the slow tight-binding of a drug to a therapeutic target. [doc]
David Perahia Characterization of large scale motions in proteins in relation to their function. [doc]
Slawomir Milewski Enzymatic targets for antifungal chemotherapy - current status and perspectives. [doc]
Jean-Louis Reymond The search for new enzymes: from antibodies to dendrimers. [doc]
David Smith Computational investigation of enzyme mechanisms: an application to the B12- independent glycerol dehydratase. [doc]
Florian Hollfelder Multiple catalytic promiscuity. [doc]
Jurgen Sygusch Reaction intermediates in glycolytic aldolases: What can they tell us? [doc]
Karl-Heinz van Pée The taming of the bleach:
enzymatically formed hypochlorite as the halogenating agent in regioselective biological halogenation. [doc]
Laurent Schio Kinases as therapeutics targets: control of selectivity in drug design. [doc]
Frank Wien DISCO a spotlight for enzymology. [doc]

Contributed Lecture Abstracts

Name Title
Pietro Gatti-Lafranconi Evolution of thermostability of a cold-active lipolytic enzyme : deep into thermal adaptation.
Chi-Ching Hwang Characterization of the proton relay system in the 3-alpha hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni : the function of Lys159 and Asn86 in proton transfer.
Matthew Macauley Multivariate free-energy relationships of O-GlcNAcase : insight into the interaction of nucleophile and leaving groups in transition state structures.
James Stivers Linked dynamic motions of DNA and uracil DNA glycosylase during extrahelical damaged base recognition.
François Talfournier Nonphosphorylating CoA-dependent aldehyde dehydrogenases : catalysis, structure and evolution.
Georg Wille Carboxylation reactions studied with caged CO2.

Poster Abstracts

Name Title Poster number
Nushin AGHAJARI * Structural studies on cold adaptation of a psychrophilic cellulase. [doc]
* Sucrose isomerase: 3D structures give insights into substrate recognition, binding and specificity. [doc]		 [1,2]
Tooba ALIZADEH Investigating the near transition state complex of beta-phosphoglucomutase, a phosphoryl transfer enzyme. [doc] [3]
Thomas BACH * Why does HMG-CoA lyase not interfere with cytoplasmic mevalonate biosynthesis in plants? [doc]
* Evidence for the plastidial origin of the isoprenyl residue used for the geranylgeranylation of a chimeric green fluorescent protein in BY-2 cells: a test system for inhibitors. [doc]		 [4,5]
Joanna BAKALA Overexpression of the prolyl oligopeptidase in human cancer. [doc] [6]
Hemalatha BALARAM Structure function studies on P. falciparum GMP synthetase. [doc] [7]
Vahe BANDARIAN Evolution of secondary metabolism in Streptomyces. [doc] [8]
Karine BASTARD Computing ligand release pathway efficiency: a comparison between robotics approach and molecular dynamics simulations. [doc] [9]
Paulo Ricardo BATISTA A new method for protein flexibility studies combining molecular dynamics simulations and normal mode analysis applied to HIV protease. [doc] [10]
Alhosna BENJDIA Anaerobic sulfatase maturating enzymes. First dual substrate radical SAM enzymes? [doc] [11]
Olivier BERTEAU Mechanistic investigations of anaerobic sulfatase maturating enzymes, Radical SAM enzymes involved in post-translational modification. [doc] [12]
Werner BESENMATTER Designed cellulase-xyloglucanase variants that withstand aggressive solutions. [doc] [13]
Didier BLANOT Characterization and substrate specificity of the Mur ligases from S. aureus. [doc] [14]
Véronique BONNET Chemo-enzymatic synthesis of phospholipidyl beta-cyclodextrins. [doc] [15]
Sandrine BOSCHI-MULLER Investigations on the mechanism of the new class of methionine sulfoxide reductase: the fRMsr from Neisseria meningitidis. [doc] [16]
Martin BRANDT Kinetic characterization of two human Acetyl-CoA Carboxylase isoforms. [doc] [17]
Dominic CAMPOPIANO Structural and functional studies of serine palmitoyltransferase. [doc] [18]
Cédric CHARRIER New family of aminopeptidase-N inhibitors. [doc] [19]
Mohamed CHIADMI X-ray structures and catalytic mechanism of urate oxydase. [doc] [20]
David CHIPMAN Acetohydroxyacid synthase and related decarboxylase-carboligase: origins of their substrate specificities. [doc] [21]
Mathieu COINCON Modelisation: structural study of the dynamic catalysis in class II FBP aldolases. [doc] [22]
Olivier COURJEAN Improving glucose oxidase for a miniaturized biofuel cell. [doc] [23]
Fabienne COURTOIS Towards directed evolution in microfluidic microdroplets on a chip. [doc] [24]
Arnaud CUTIVET New biomimetic material for enzyme inhibition and recognition obtained by molecular imprinting. [doc] [25]
Colette DAUBNER Mobile loop containing residues 423-426 of tyrosine hydroxylase optimizes tyrosine binding and substrate specificity. [doc] [26]
Elisabeth DAVIOUD-CHARVET Proposed half-site reativity towards NADPH binding for P. falciparum glutathione reductase in vivo. [doc] [27]
Hélène DEBAT Structural analysis of T. xylanilyticus GH-51 arabinofuranosidase: from the sequence to the crystal structure. [doc] [28]
Geneviève DESCHUYTENEER      Intein splicing in the periplasmic space of E. coli. [doc] [29]
Joëlle DUBOIS Synthesis and biological evaluation of constrained analogues of farnesyl pyrophosphate. [doc] [30]
Anne DUE Molecular basis of catalytic promiscuity of the bi-substrate enzyme phosphoribosyl isomerase A from M. tuberculosis. [doc] [31]
Laure DUFAU Dimer disruption and monomer sequestration of HIV-1 protease by rationally designed interface peptidomimetics: mechanistic proofs. [doc] [32]
Heli ELOVAARA Characterization of the endothelial oxidase AOC3 D386N mutant. [doc] [33]
Stéphane EMOND Cloning purification & characterization of a thermostable amylosucrase fro D. geothermalis [doc] [34]
Paul FITZPATRICK Kinetic isotope effects as mechanistic probes of flavoprotein oxidases. [doc] [35]
Nicolas FLOQUET On the use of normal mode analysis in computer assisted drug design: applications to enzymes and receptors. [doc] [36]
Johanna FORET Design, synthesis, kinetic & theoretical structural studies of substrates and inhibitors of phosphomannose isomerase. [doc] [37]
Patrick FRANTOM Identification of a discrete allosteric pathway in a multidomain enzyme provides evidence for a specific inhibition mechanism. [doc] [38]
Leonid GAIDUKOV Directed evolution and mechanistic studies of serum paraoxonase. [doc] [39]
Pietro GATTI LAFRANCONI Evolution of thermostability of a cold-active lipolytic enzyme: deep into thermal adaptation. [doc] [40]
Mahmoud GHANEM Loop-tryptophan human PNP reveals sub-millisecond protein dynamics. [doc] [41]
Hicham GOUZI Partial purification & characterization of tyrosinase from A.bisporus. [doc] [42]
Thomas HARRIS Kinetic mechanism of S6K1 Protein Kinase. [doc] [43]
Florian HOLLFELDER Formation of an MgF3-phosphate transfer transition state analogue in an enzyme active site. [doc] [44]
Fanglu HUANG Site-directed mutagenesis and X-ray crystallographic analyses of wild type and mutant fluoroacetyl-coenzyme A thioesterase FlK from S. cattleya. [doc] [45]
Chi Ching HWANG Characterization of the proton relay system in the 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from C. testosteroni. [doc] [46]
Dirk IWATA REUYL Biochemical and structural investigation of a new prokaryotic specific GTP cyclohydrolase I enzyme family. [doc] [47]
Stefanie JONAS Structural and kinetic analysis of a highly catalytically promiscuous enzyme with seven activities. [doc] [48]
Pierre Alexandre KAMINSKI Characterization of a new mammalian enzyme: deoxynucleoside 5-monophosphate N-glycosidase. [doc] [49]
Bachra KHETTAL Kinetic properties of delta5,3-ketosteroid isomerase from Comamonas testosteroni in organized heterogenic media. [doc] [50]
Krzysztof KUR Homocitrate synthase as a novel target for antifungal agents. [doc] [51]
David KWAN Origin of stereospecificity of enoylreductase domains in modular polyketide synthases. [doc] [52]
Yanyan LI Biosynthesis of quinoline alkaloids Aurachins from S. auranciaca Sg a15. [doc] [53]
Matthew MACAULEY Multivariate free-energy realtionships of O-GlcNAcase: insight into the interaction of nucleophile and leaving groups in transition state structures. [doc] [54]
Stéphane MANN M. tuberculosis DAPA aminotransferase, a potential therapeutic target against tuberculosis. [doc] [55]
Sandrine MARIANO Evidence of the new allose-6P isomerase activity of type B ribose 5-phosphate isomerase from E. coli. Synthesis and evaluation of specific inhibitors. [doc] [56]
Olivier MARTIN  Novel UDP-Galf and Galf-disaccharide mimics: inhibitors of the biosynthesis of mycobacterial cell wall galactan? [doc] [57]
Valery MATHIEU Towards engineering allosteric regulation into a circularly permuted TEM-1 beta-lactamase. [doc] [58]
Annick MEJEAN Biosynthesis of anatoxin-a, a neurotoxin of cyanobacteria: a study combining genomic, biosynthetic and molecular approaches. [doc] [59]
David MERKLER The electronic nature of the peptidylglycine alpha-amidating monooxygenase transition state: evidence for a radical mechanism. [doc] [60]
Chiara MICALELLA Immobilisation of catechol 1,2-dioxygenase in silica gel as a potential bioremediation device. [doc] [61]
Slawomir MILEWSKI Mechanism of inactivation of yeast glucosamine-6P synthase by epoxyde containing glutamine analogues. [doc] [62]
Susan MILLER Is the Lys-Glu salt bridge of disulfide reductases just a salt bridge? [doc] [63]
Ralf MORGENSTERN Apparent positive and negative cooperativity towards glutathione in microsomal glutathione transferase 1. [doc] [64]
Hélène MUNIER LEHMANN  Inhibitors thymidine monophosphate kinase in M. tuberculosis. [doc] [65]
Alexey NIKULIN L-methionine gamma-lyase: structural studies of a potential drug target. [doc] [66]
Jitka NOVOTNA LmbB2, a unique tyrosine hydroxylase operating in Lincomycin biosynthesis. [doc] [67]
Yvonne NYMALM-REJSTRÖM Human TPQ monoamine oxydases: in search for a specific ligand. [doc] [68]
Ann Marie O'DONOGHUE Proton transfer catalyzed by methylglyoxal synthase: contribution of substrate phosphodianion group to catalysis. [doc] [69]
Folasade OLAJUYIGBE  Biocrystallographic study on drug resistant variants of HIV protease with new inhibitors. [doc] [70]
Luis OLGUIN-CONTRERAS Cell-based enzymatic assays in microdroplets. [doc] [71]
Stefano PARAVISI Studies on M. tuberculosis glutamyl-tRNA synthetase. [doc] [72]
Eugenia PENNACCHIETTI E. coli glutamate decarboxylase: role of the substituted aldamine in the catalytic and spectroscopic properties of the enzyme. [doc] [73]
Olivier PLOUX E. coli cyclopropane fatty acid synthase as a model for M. tuberculosis cyclopropane synthases: mechanistic and inhibition studies. [doc] [74]
Dean REA Molecular enzymology of aromatic degradation. [doc] [75]
Michèle REBOUD-RAVAUX Development of non-covalent inhibitors of the 20S proteasome: further structural variations and effects on cellular models of the disease. [doc] [76]
Jim REID Metal-ion chelation in the active site of magnesium chelatase. [doc] [77]
Magali REMAUD SIMEON Engineering transglucosidases regiospecificity for programmed chemo-enzymatic synthesis of complex bacterial carbohydrates. [doc] [78]
Moez RHIMI Human cytosolic 5’-nucleotidase: a target of therapeutic interest with a double catalytic function. [doc] [79]
Nigel RICHARDS A HMQC-based NMR assay for investigating ammonia tunneling in glutamine-dependent amidotransferases: studies of human asparagine synthetase. [doc] [80]
Xavier ROUSSEL Evidence for the formation of a covalent thiosulfinate intermediate with Peroxiredoxin along the catalytic mechanism of Sulfiredoxin. [doc] [81]
Ludovic SAUGUET Cyclopeptide synthases, a new family of peptide bond forming-enzymes involved in the biosynthesis of diketopiperazine metabolites [doc] [82]
Laure SELME The periplasmic PilB protein from N. meningitidis: regeneration mechanism of the methionine sulfoxide reductase activity [doc] [83]
Aloysius SIRIWARDENA Is natural product mimicry an effective strategy for the design of inhibitors of oligosaccharide-processing enzymes? [doc] [84]
Lucie SKARYDOVA Purification & characterization of human membrane-bound carbonyl reductases. [doc] [85]
Claire STINES-CHAUMEIL PQQ glucose dehydrogenase engineering for application in biofuel cells and glucose sensor. [doc] [86]
James STIVERS Linked dynamic motions of DNA and uracil DNA glycosylase during extrahelical damaged base recognition. [doc] [87]
Erick STRAUSS Design, synthesis and characterization of mechanism-based inhibitors of CoA disulfide reductase. [doc] [88]
François TALFOURNIER Nonphosphorylating CoA-dependent aldehyde dehydrogenases: catalysis, structure and evolution. [doc] [89]
Charles TELLIER Converting glycosidase into transglycosidases by directed evolution. [doc] [90]
Manuela TOSIN Trapping of polyketide biosynthesis intermediates via a novel malonyl-CoA analogues. [doc] [91]
Marielle VALERIO LEPINEC A direct approach of the E. coli glucosamine-6P synthase by ITC and DSC. [doc] [92]
Thomas VARGUES Studies of the enzymes involved in the modification of lipid A with the 4-amino-4-deoxy-L-arabinose in B. cenocepacia. [doc] [93]
Daniel VEGA MENDOZA Cloning, expression, purification and characterization of a highly thermostable dehydrogenase of Thermus thermophilus HB27 . [doc] [94]
Olivia VERGNOLLE Dehydratase domains of the borrelidin producing polyketide synthase. [doc] [95]
Benoît VILLIERS Substrate promiscuity and directed evolution of a non ribosomal peptide synthase adenylation domain. [doc] [96]
Mikael WIDERSTEN Plant epoxide hydrolase StEH1; structure and function. [doc] [97]
Georg WILLE Carboxylation reactions studied with caged CO2. [doc] [98]
Gabrielle WORONOFF A new high throughput screening method for evolving penicilline acylase specificity. [doc] [99]
Sandra WYDAU Universality of D-aminoacyl-tRNA deacylase in the living world. [doc] [100]
Larry XI Achieving accelerated re-activation of AmpliTaq Gold through site-directed mutagenesis. [doc] [101]